King James V of Scotland, son of Margaret Tudor, and Queen Marie de Guise, parents of Mary, Queen of Scots. Marie was the second wife of King James V. She served as regent of Scotland in her daughter's name from 1554 to
1575 Portrait of a royal lady traditionally identified with Mary, Queen of Scots.The Devonshire Collection, Traditionally attributed to Federico Zuccari (or Zuccaro) and after to Alonso Sánchez Coello.
Queen Anne Stuart Of England was the sister of Queen Mary II. Despite 17 pregnancies, Anne died without surviving children and was the last monarch of the House of Stuart. She was succeeded by her second cousin George I of Hanover.